The structural performances including shear-strength, stiffness, and failure mode of structural adhesives with two different curing schedules at room and high temperatures were investigated. The test results showed that the high temperature curing on the structural adhesive after room temperature curing can improve the value of glass transition temperature Tg by about 30 ℃. Moreover, the shear performances of the structural adhesives were deteriorated with the increase of the temperature, and the maximum degradation rate occurred within the temperature range of Tg±20 ℃. It is indicated that the glass transition temperature Tg was critical to the shear behavior of the structural adhesive at the high temperature. In a whole, the high temperature curing can improve the value of glass transition temperature, and it is helpful to delay the degradation of shear performance at the elevated temperature. Based on the test results, the normalized relationship between the shear performances and temperature were eventually proposed, in which glass transition temperature was considered.