Based on the concept of ductile joints, a prefabricated concrete beam-column ductile joint is proposed, in which low-yield-point and high ductility rods and steel brackets are used for linkage. A quasi-static cyclic loading test was carried out to evaluate the seismic response of the new prefabricated ductile joint. Three full-scale specimens were designed and manufactured, including two new prefabricated ductile specimens with different forms of ductile linkage and one cast-in-place connection specimen for comparison. The failure modes, hysteretic performance and bearing capacity of the three specimens were compared and analyzed. The new prefabricated ductile joint had about 43% higher yield displacement and about 14% higher peak load than those of the cast-in-place comparison joint, and both ductility and bearing capacity were improved. Moreover, as an integral ductile rod anchor block was embedded in the core area of the joint, the joint showed better overall compatible deformation ability, higher displacement ductility and bearing capacity.