The removal of phosphate from aqueous solution using the anion-exchange membrane was investigated without supplying electrical potential based on Donnan dialysis principle. The results showed that the removal efficiency of phosphate achieved 70.0 % under the conditions of influent phosphate of 2.0 mg/L(0.064 5 mmol/L), the NaCl concentration of 0.1 mol/L in the receiver solution, the flow rate of the feed solution of 5.0 mL/min. Decreasing the initial pH of the feed solution to 3.0, the phosphate concentration outlet in the feed solution was much higher than that with the initial pH of 5.0~9.0,and the removal efficiency of phosphate was only 43.5 %. In the tested concentration range, the accompanying anions (Cl-，HCO-3 and SO2-4) had little effect on the removal of phosphate. Using Na2SO4 solution as the receiver phase, the removal of phosphate greatly reduced. Decreasing the NaCl concentration in the receiver solution had little influence on the transport of phosphate, but the concentration of phosphate in the receiver solution decreased significantly. Increasing the flow rate of the feed solution from 2.5 mL/min to 10.0 mL/min , the removal efficiency of phosphate decreased obviously from 84.3 % to 61.1 %.