In order to improve dynamic performance and reduce the vibration produced by high-speed industrial sewing machine, a method of dynamic balance and optimization was applied to improve the key mechanisms, the puncturing and stirring mechanism, of the sewing machine. A dynamic model of puncturing and stirring mechanism was built in the dynamic simulation of mechanical system. The sensitivity of each variable on the analysis target- RMS of the vibration force and vibration moment was analyzed. Response surface approximate models, which express the approximate relationship between the design parameters and the analysis target, were established and verified. The reasonable values for weight coefficients were assigned and confirmed to build the optimal mathematical model. The optimization of sewing machine was carried out by means of optimizing parameters, such as mass and placement of the mass centroid of mechanism, based on MIGA. The vibration force and vibration moment of the puncturing and stirring mechanism were significantly improved after optimization. The optimization results indicate that these methods have high accuracy and validity, which has reference value for solving the vibration and noise of complex mechanical systems such as high-speed industrial sewing machine.