Surface properties of tissue engineering scaffold play important roles in cell adhesion and other biological processes such as cell proliferation,differentiation. In this study,the surface modification of Poly (3 hydroxybutyrate co 3 hydroxyvalerate) (PHBV) was performed in order to improve the biological activity of material surface. PHBV nanofibrous scaffold was firstly made by electrostatic spinning method,introducing free amino groups in the PHBV surface through 1,6 hexanediamine,which provided reaction sites for the further immobilization of hyaluronic acid (HA) macromolecules. The morphology observed by SEM showed that PHBV nanofibrous scaffolds prepared by electrostatic spinning method have smooth surfaces without obvious beads defects and their fiber diameters uniformly distribute. FTIR analysis proved that 1,6 hexanediamine and hyaluronic acid modified PHBV fibrous scaffolds were successfully achieved. The ninhydrin determination results showed that the surface amine group density,which increased with the extending of aminolysis time,reached its maximum at 50 minutes. Furthermore,there is no significant difference on the morphology of the scaffolds before and after modification by statistic comparison. The water contact angle significantly decreased after the immobilization of hyaluronic acid,indicating that the hydrophilicity was obviously improved after surface modification with the hydrophilic natural biological macromolecules. The Alamar Blue assay manifested that hyaluronic acid modified PHBV nanofibrous scaffolds could significantly promote the chondrocytes proliferation in vitro. Therefore,the hyaluronic acid modified PHBV nanofibrous scaffolds are potential for application in cartilage tissue engineering.