朱剑1,蒋雪梅1,钟军1,段翼远1,邓林红1,2.p(nBA-DEAEMA)的合成及其作为人工气管内表面修饰材料的初步研究[J].湖南大学学报:自然科学版,2017,44(12):108~115
p(nBA-DEAEMA)的合成及其作为人工气管内表面修饰材料的初步研究
Preparation of p(nBA/DEAEMA) Copolymer Brushes and ItsFeasibility Study as Surface Modification for Inner Face ofArtificial Trachea
  
DOI:
中文关键词:  p(nBA-DEAEMA)  乳液  纳米粒子  膜材料  生物安全性
英文关键词:p(nBA-DEAEMA)  copolymer emulsion  nanoparticles  cilia-like membrane  biosecurity
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作者单位
朱剑1,蒋雪梅1,钟军1,段翼远1,邓林红1,2 (1.重庆大学 生物工程学院重庆 400030
2.常州大学 生物医学工程与健康科学研究院江苏 常州 213164) 
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中文摘要:
      为制备p(nBA-DEAEMA)乳液及表面具有纤毛样结构的共聚物膜材料,采用半连续乳液聚合法合成p(nBA/DEAEMA)乳液,再用Velcro成型工艺将纯化干燥的产物制备成纤毛样膜.采用傅里叶红外光谱(Fourier Transform Infrared Spectrometer,FT-IR)、核磁共振光谱(Nuclear Magnetic Resonance Spectroscopy,1HNMR)、凝胶色谱(Gel Permeation Chromatography,GPC)、粒径分析、扫描电子显微镜(Scanning Electron Microscope,SEM)、静态接触角测定、示差扫描量热分析(Differential Scanning Calorimetry,DSC)及力学分析对材料进行详细表征.最后用浸提液实验对材料的生物安全性进行了初步评价.结果显示:FT-IR和1HNMR表明目标产物结构正确.共聚物乳液的多分散系数(polydispersity,PDI)为0.385,平均粒径(Z-average)为46.48 nm.DSC结果显示共聚物的玻璃化温度(glass transition temperature,Tg)和粘流温度(viscous flow temperature,Tf)分别为33.2 ℃和47.7 ℃.纤毛样膜对水的接触角为102.3°,表面自由能13.81 J·m2.SEM结果显示共聚物粒子大小为40~50 nm,纤毛样层高度为5~10 μm.细胞实验结果显示材料对大鼠气道上皮细胞(bronchial epithelial cells,BECs)具有可接受的生物安全性.
英文摘要:
      p(nBA/DEAEMA) nanoparticles of emulsion and polymeric cilia-like membranes were prepared. Two monomers of n-butyl acrylate (nBA) and 2-(diethylaminoethyl methacrylate) (DEAEMA) were polymerized by semi continuous emulsion polymerization method,and the cilia-like membranes were prepared by attaching a Velcro-like surface to partially coalesced films. Subsequently,the prepared materials were characterized by FT-IR,1HNMR,GPC,SEM,DSC combined with particle size analysis,static contact angle measurements and mechanical analysis. Finally,BECs from SD rats were used to characterize the biosecurity of the material. Correct structure of copolymer was also confirmed by FT-IR and 1HNMR. The particle size analysis of the copolymer emulsion was PDI 0.385 and Z-average was 46.48 nm,while Tg and Tf for copolymer was 33.2℃ and 47.7℃,respectively. The contact angle of polymeric films was 102.3° with water,and the surface free energy was 13.81 J·m2. Meanwhile,SEM photos showed the size of particles was 40 to 50 nm,and the height of cilia-like layer was about 5 to 10 μm. The biosecurity of this material was eventually verified by cell experiments. The copolymer cilia-like membranes can be used as a surface modification material for artificial trachea.
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