A recombinant protein expression vector pET15b-R9-FOXM1(1-234aa) was constructed and transformed to E. coli in order to generate a strain expressing R9-FOXM1(1-234aa). The recombinant protein R9-FOXM1 (1-234aa) (R9-FOXM1(1-234aa)) was isolated at a large scale through His-tag affinity chromatography. The purity of the purified protein reached 90%. Moreover, MTT assay was used to test the effect of R9-FOXM1(1-234aa) on cells, and the test results showed that R9-FOXM1(1-234aa) caused the cell death of different types of cancer cells with a half lethal dose around 2 mm。 The results also demonstrated that R9-FOXM1(1-234aa) suppressed the proliferation of cancer cells and may be considered as a potential angent for anti-cancer in the future.