陈耀宁1,2,汪元南1,2,黎媛萍3,陈艳容1,2,苟宇1,2,朱福造1,2,张道利1,2,刘耀1,2.液态培养条件下烟曲霉去除Cd的研究[J].湖南大学学报:自然科学版,2017,44(12):127~134
液态培养条件下烟曲霉去除Cd的研究
Study on Removal of Cd by Aspergillus fumigatus in Liquid Culture
  
DOI:
中文关键词:  烟曲霉  谷胱甘肽  液体培养    去除
英文关键词:Aspergillus fumigatus  glutathione  liquid culture  cadmium  removal
基金项目:
作者单位
陈耀宁1,2,汪元南1,2,黎媛萍3,陈艳容1,2,苟宇1,2,朱福造1,2,张道利1,2,刘耀1,2 (1.湖南大学 环境科学与工程学院湖南 长沙 4100822.环境生物与控制教育部重点实验室(湖南大学)湖南 长沙 4100823.湖南城市学院 市政与测绘工程学院湖南 益阳 413000) 
摘要点击次数: 93
全文下载次数: 22
中文摘要:
      研究了一株高耐Cd的烟曲霉在实验室摇瓶培养条件下对不同浓度Cd的去除效果,测定了其在去除过程中菌株产谷胱甘肽 (GSH) 的变化.结果表明,在重金属Cd浓度为50 mg/L时,烟曲霉去除Cd效率最佳,达到55.5%的去除率,其产生的GSH浓度达到了0.89 μmol/mg.研究比较了不同环境条件下烟曲霉对Cd的去除效率,发现在温度30 ℃,pH 5.0,加液量120 mL,转速100 r/min,接种量0.4 mL(1.0×107 CFU/mL)的培养条件下,烟曲霉能达到最佳去除Cd的效率.通过这种培养条件的优化,烟曲霉较初始条件下对50 mg/L Cd的去除率提高了29.8%,培养体系中GSH浓度增加了0.42 μmol/mg.谷胱甘肽过氧化物酶(GPX)和谷胱甘肽还原酶(GR)的变化表明烟曲霉在优化的液态生长环境下可以较好去除培养体系中的重金属Cd,其产生的GSH对Cd的去除有促进作用,其解毒机制可能是依靠体内GPX和GR将应激产生的氧化性物质去除来实现.
英文摘要:
      The removal efficiency of a Cd-resistant Aspergillus fumigatus under different concentrations of Cd in laboratory flask culture was studied,and the changes of glutathione (GSH) production in the strain were determined. The results showed that the removal rate of Cd was 55.5% when the concentration of Cd was 50 mg/L,and the concentration of GSH was 0.89 μmol/mg. The comparison of Cd removal efficiency by Aspergillus fumigatus under different conditions showed that the optimal condition was 30 ℃,pH value of 5.0,add fluid volume of 120 mL,speed of 100 r/min,and inoculation amount of 0.4 mL (1.0×107 CFU/mL). Under the optimal condition,the removal rate of Cd was 29.8% higher than that under the initial condition,and the GSH content in the culture system was increased by 0.42 μmol/mg. The changes of glutathione peroxidase (GPX) and glutathione reductase (GR) indicated that Aspergillus fumigatus could remove the heavy metal Cd in the culture system under the optimized liquid growth conditions,GSH could promote the removal of cadmium,and its detoxification mechanism could be achieved by removing the oxidative substances produced by stress by GPX and GR in vivo.
查看全文  查看/发表评论  下载PDF阅读器
关闭