Study on Effect of FT Gene on Root Hair Development in Arabidopsis
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摘要:
为进一步研究成花素（FLOWERING LOCUS T，FT）基因对植物营养生长的影响，通过结合qRT-PCR和生理学实验的方式对FT过表达及突变体植株进行比对分析.研究发现，FT过表达植株与野生型相比，其根毛短且稀疏，且具有分叉型、膨大型、波浪型等极性生长缺陷表型；相反，突变体ft-10的根毛相对长且密集，略优于野生型，无极性生长缺陷表型. 通过实时定量PCR分析与根毛起始伸长过程相关基因KOAJK、SCN1、RHD2、LRL3、RSL4、RHD6的表达量， FT过表达植株显著低于野生型，而突变体ft-10略高于野生型. 结果表明， FT基因在根毛起始伸长过程中起负调控作用，影响根毛的极性生长及数量分布. 本研究增加了对FT基因在营养生长过程中功能的认识，同时也为深入研究根系发育的分子机理奠定了基础.
Abstract:
The FT （FLOWERING LOCUS T） overexpression and mutant plants were analyzed by quantitative PCR and physiological assays to further study the effect of FT gene on the plant vegetative growth. The results showed that the FT overexpression lines had shorter and sparser root hairs with abnormal phenotypes such as branch, ballooned and wavy types when compared with the wild type. On the contrary，ft-10 had longer and denser root hairs with normal phenotypes when compared with wild type. Quantitative analysis of the gene expression including KOAJK、SCN1、RHD2、LRL3、RSL4、RHD6 related to root hair initiation elongation showed that the FT overexpression lines were significantly lower than those of the wild type, while ft-10 were slightly higher than those of the wild type. The results indicate that the FT gene plays a negative role in the initial elongation of root hairs and affects the polar growth and quantitative distribution of root hairs. This study promotes the understanding of the FT function in the vegetative growth process，and also lays the foundation in research of the root development mechanism.
