The existing connections for glulam/bamboo-concrete composite（BCC） beam，including notched connection and dowel connection，exhibit performance deficiencies. Therefore，a new kind of connection，named as reactive powder concrete（RPC）-steel composite connection，was presented for prefabricated BCC beams in this paper，in which steel screw with an axial hole was covered by a layer of RPC. Total 6 groups of specimens were performed by push-out tests. The major mechanical properties such as the load-slip curves，shear capacity and shear stiffness were measured and then evaluated. The results showed that the RPC coat significantly improved the shear stiffness of composite connection and increased the shear capacity in a certain degree，while the shear capacity was mainly decided by the diameter of steel screw. Compared with the notched connection and dowel connection，the proposed connection has higher carrying efficiency per unit area and higher shear stiffness，respectively. Therefore，the RPC-steel composite connection combines the merits of high shear stiffness and high ductility from two conventional connections，and it is fit to prefabricated construction. Based on the test results，the design size of the proposed connection was also provided.