To study the influence of the disturbance-flow fan on the passengers' thermal comfort on the subway train, the new disturbance-flow fan type B subway car occupants was taken as the research object. The human body's physiological temperature regulation model with numerical simulation loading Stolwijk was used to study the cabin crew's human thermal comfort by combining the airflow discomfort index and Berkeley thermal comfort evaluation model. Experiments verified the accuracy of the simulation model. The influence of the loading disturbance-flow fan on the human body microenvironment and various indexes were analyzed when the air supply temperature of the air conditioner in the compartment was 20 ℃. The difference of occupant index in different frequency perturbation field was analyzed. The results show that the disturbance-flow fan can improve the flow velocity and uniformity and the air distribution in the compartment, and it can optimize the human microenvironment's heat flow field. After loading the disturbance-flow fan, the occupant's overall thermal sensation is decreased by 7.3%, and the thermal comfort is increased by 0.76%. Within a certain range, the human body's thermal comfort decreases as the disturbance frequency increases, and the optimal disturbance field function frequency is 2.75 times /min.