The fatigue life of polypropylene fiber reinforced concrete (PFRC) under constant-amplitude fatigue uniaxial compression at multiple levels was investigated. A total of 180 specimens were made taking into consideration of two factors: tested with polypropylene fiber aspect ratios and volume fractions and aspect ratios taken into consideration, with an emphasis on the fatigue failure mode, fatigue deformation behavior, and a fatigue equation is established. The results showed that three failure modes of PFRC can be classified: splitting failure, wedge failure and shear failure. Meanwhile, the failure section of the specimen could can be divided into three zones: fatigue source zone, fatigue crack stable propagation zone and instantaneous fracture zone. In addition, the PFRC specimen with an aspect ratio of 280 and a volume fraction of 0.2% provides the highest fatigue strength among all the designed specimens, and its fatigue strength is increased by up to 33.83% when compared with plain concrete. Finally, a unified fatigue equation was developed in consideration of fiber parameter, which can concisely describe the relationship between stress level, fatigue life and fiber parameter at a certain failure probability. The research provides a reference for the future development of design specifications and analysis of PFRC structures under cycle dynamic loads.