The existing studies on the influence of the environmental temperature on the natural frequency characteristics are usually carried out for a specific bridge， and their applicability is limited. As concrete beam-type bridge is the most widely used bridge type， this paper deduces the general iterative formula of the relationship between natural frequency and temperature for concrete beam-type bridges and verifies it via numerical examples and laboratory tests. The results indicate that the influence of environmental temperature on the natural frequency cannot be ignored， and the proposed general iterative formula can effectively consider the environmental temperature influence and can be used to calculate the natural frequency of concrete beam-type bridges under actual temperature.