Multiple alkynyl linear polyurethane resins(MAPU) were synthesized by polyaddition reaction using polyethylene adipate diethylene glycol(PADG), isophorone diisocyanate(IPDI) and 1,4-butynediol(BD). The molecular structures of resins were characterized by Fourier transform infrared spectra and Raman spectra. Series of sulfhydryl-alkynyl UV curing polyurethane films(F-SAPU) and coatings(C-SAPU) were prepared using trimethylolpropane tris(3-mercaptopropionate)(TTMP) as a crosslinking agent. The synthetic and curing process about sulfhydryl-alkynyl UV curing resins were designed according to formulation parameters such as chain extension parameters(Ep), hard segment content(Hc%), curing parameters(Cp) and photoinitiator dosage(Pi%). The results showed that the tensile strength and elongation at break of F-SAPU were flexibly adjustable from 0.48 MPa to 5.32 MPa and 106% to 172%, respectively, and the glass transition temperature was in the range of –10.1°C to 26.9°C, when changing the formulation parameters. In addition, the effect of Hc% on the performance of C-SAPU was explored. When Hc% was 45%, the coating had optimal performance.