t-test method was introduced, including the principles of the method, the evaluation of characteristic ratios and the identification principle. An example was given. The identification of two different crude oils, as well as one crude oil and its weathered oil (7 days), was conducted with the t-test. In this t-test method, oil fingerprinting can be directly identified by neat graphic traits, rather than by using fussy spectra or abstract data in traditional methods, which would decrease the subjectivity and artificial distinctions of visual spectra. t-test is more reliable in presenting the quantitative index. This will be useful for the standardization and popularization of oil fingerprinting identification method. The limitations of this t-test method for oil fingerprinting identification were also given.