The genetic evolutionary structural optimization (GESO) is used for single loading case study, but it still needs to be improved in practical engineering.In this paper, Separated elements model GESO was used to design reinforcement layout of the reinforced concrete (RC) members under complex elements stress states and multiple loading cases in order to fulfill the needs of engineering application.Separated elements model GESO involves the nonlinear analysis of ANSYS finite element software as a platform, in which solid65 element is chosen as the concrete element, while link10 element is regarded as the reinforcement element herein.Separated elements model GESO is further verified by considering deep beams and shear walls with openings as the numerical examples.The reinforcement layout of RC members under complex stress states can be optimized under multiple loading cases including loading combination.The influence of subjective factors has been dramatically reduced with intuitive reinforcement layout results, which are in accordance with the stress mechanism and will not lead to centralized reinforcement layout.The application of separated elements model GESO can save steel consumption by comparing with elastic stress design method, since the utilization efficiency of reinforcements is also improved.