The physical and chemical properties of concrete specimens (C35) were carefully observed under high temperature (300 ℃, 400 ℃, 600 ℃, 800 ℃)and then put into cool water. After that, the concrete specimens were used for uniaxial compression test. Finally, the failure modes, strength, elastic modulus and other mechanical properties of concrete specimens were obtained. Based on the test results, the temperature and water cooling damage constitutive model of concrete materials were constructed. The four unknown parameters of the constitutive model were recognized with the differential evolution algorithm. The theoretical arithmetic stress-strain curve and experiment curve are greatly consistent. So the study result illustrates the damage constitutive model is well applicable for concrete under high temperature.