Wind tunnel tests were carried out to measure the wind pressure on the internal surface of a 220 m-high super large cooling tower, and then analysis on the three-dimensional effect of internal pressure was conducted, and finally, simplified methods for obtaining the design value were investigated. The analysis results indicate that the wind loads on the internal surface of the cooling tower behave with remarkable three-dimensional effect, and they are not strictly uniformly distributed along both the hoop and the meridional direction. This nonuniformity is particularly serious when the tower is in the construction period. The size and the distribution of the internal pressure have some influence on the wind-induced response, but because the outer pressure plays a dominant role in the wind-induced response of cooling tower, the contribution of the internal pressure to the response is small. So the design value of internal pressure can be simplified as constant -0.50, which is proved to be very safe for the structure. The results can provide reference for both the design of 200 m high super-large cooling tower and our specification revision.