In order to address the matching optimization of one bus with variable stiffness suspension, traditional suspension matching methods and processes were firstly summarized and then the virtual matching optimization method was proposed based on Computer Aided Optimization (CAO) technology. The corresponding matching process was also given, in which the virtual dynamic optimal model of the vehicle was established, the matching optimization was programmed and the optimization goal was also set. For an accurate virtual model, the mechanical characteristics of the bus' tires were tested and the inertial parameters of the bus' sprung mass were also measured under unloaded and full loaded states. The matched suspension parameters included the torsion stiffness of front torsion bar, the pre and post damping curve coefficients, the torsional stiffness of pre and post stabilizer bars, the two-stage variable stiffness of rear suspension and the radial stiffness of the leaf spring bushing. When virtually matching, the vehicle performance under two kinds of loaded states (unloaded and full loaded) was considered respectively. Then, the matched results of the variable stiffness suspension were obtained. The suspension samples were made according to the matched results, and the comparative trials of vehicle were done in an automotive proving ground to evaluate the optimization effect. The results show that the proposed virtual matching optimization method is effective and feasible for matching the variable stiffness suspension parameters.