DHHC-type zinc finger proteins are involved in the palmitoylated modification of intracellular proteins and play important roles in the growth and development of cells and individuals. OsDHHC 13 is a typical DHHC-type zinc finger gene. Bioinformatics analysis showed that the OsDHHC 13 promoter region contains some stress and light response elements, and its encoded protein is a typical membrane protein with four transmembrane domains. When the OsDHHC 13 overexpressing lines were suffered from oxidative stress with H2O2, the roots of overexpressing seedlings were significantly longer than those of wild-type, indicating that OsDHHC 13 can remove the inhibition of root growth by H2O2 and improve the ability of resistance to oxidative stress. Subsequently, analysis of H2O2 contents found that the H2O2 contents in the roots of overexpressing seedlings were markedly lower than that in wild type. qRT-PCR analysis further revealed that the transcriptional levels of enzyme genes related to H2O2 elimination increased significantly, suggesting that OsDHHC 13 can promote the expression of enzyme genes related to H2O2 elimination and regulate the homeostasis of endogenous H2O2 in plants. In a whole, present study preliminarily proved that the zinc finger protein gene OsDHHC 13 positively regulates the ability of resistance to oxidative stress.