Contrary to the current backward status of the agricultural environmental information organization in the Dongting lake area, the database optimization foundation theory of ArcSDE+SQLServer2000 was adopted. Through reorganization analysis and quality control with various agricultural data source of the Dongting Lake region in a standardization type, this paper expounded the method of designing and realizing comprehensive agro-environment information database founded on Optimizing Theory. The results have shown that the optimizing theory provides complete mechanisms of data storage and management for regional agro-environmental information database, and obtains optimized implementation in many core technologies, such as massive data organization, storage of Complex Format data, data interoperable and so on. This provides a relatively standardized and more efficient theory and technical guidance for the construction of regional agro-environmental database.