An idea model of polycystic kidney cells CRL2830 with angiogenesis factor receptor 2 (VEGFR2) low expression was established by applying 3-D culture and shRNA technology, which can be applied to reveal the role of VEGFR2 in polycystic kidney disease. The change of biological characteristics in this cell line was observed,and the results have shown that, although VEGFR2 low expression does not affect cell growth and invasion, it can result in the volume increase of cell and nucleus and the enhancement of cell aggregation. Furthermore, the low expression of VEGFR2 can not only inhibit cyst growth but also efficiently induce early cell apoptosis in 3-D culture cells, compared with parental cells.