The splitting fatigue tests of three types of AC25 asphalt concretes under freeze-thaw and without frozenthaw condition were carried out. The gradations of aggregate in asphalt concretes were coarse, medium and fine. The stress and strain fatigue equations were obtained with the result of the tests. The effect of water, temperature, load and their coupling action on the fatigue life were studied. The results are as the following: the fatigue life of asphalt concrete is in good relation to its split strength, the lower split strength of the asphalt concrete, the lower fatigue life. According to fatigue life, the gradations of different asphalt concretes from best to worst is as follows: rough, fine and medium. The effect of temperature on the fatigue life of asphalt concrete is not obvious in strain control mode test, and the strain fatigue equations at different temperatures can be given with a single equation, which interprets that the process of strain fatigue for asphalt concrete has nothing with temperature. The fatigue life of unfrozenthaw coarse asphalt concrete reduced in a large scope as the increases of temperature. When subjected to a freezethaw cycle, the fatigue life for different types of asphalt concretes reduce about 10% to 35% in the same stress ratio, while it reduce about 75%～80% in the same stress level.