Bioleaching heavy metal from mine tailings has been widely accepted. In this study, the bacteria indigenous Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans isolated from an acidic mine tailings dam were grown and acclimated in presence of mine tailings and then used as bioleaching bacteria to solubilize the mine tailings. After 12 days of bioleaching, the laboratory results of the experiment demonstrated that the indigenous bacteria had high value of bioleaching. And the percentages of copper and zinc solubilized into the leaching solution from tailings basically increased with the optimal bacteria. The percentage of Zn solubilized is 98.12% when indigenous Acidithiobacillus ferrooxidans are used as bioleaching bacteria. The percentage of Cu solubilized is 88.75% when the mixture of indigenous Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans are used as bioleaching bacteria.