Strains R4-1, R4-2, R4-3, R4-5, H4-3 were obtained by separation and purification experiment from poly-containing wastewater, Sodium Alginate-PVA as embedding medium, immobilized experiment research of mixed bacteria group were studied by Embedding fixed method . The volume ratio of wet bacterium to embedding medium, the mass fraction of calcium chloride, the mass fraction of boric acid and the immobilized time on degrading poly-containing wastewater were investigated by experiment of single factor and orthogonal experiment. The optimum process conditions of degrading poly-containing wastewater by immobilize microorganism are as follows: the mass fraction of sodium alginate is 4%; the mass fraction of PVA is 4%; the volume ratio of wet bacterium to embedding medium is 1∶1; the mass fraction of calcium chloride is 2%; the mass fraction of boric acid is 3%; the immobilize time is 4 h immobilization in calcium chloride solution and then 20 h immobilization in boric acid solution. The main factor affecting microbial immobilization was volume ratio of wet bacterium to embedding medium. Experiment subjoined by optimum process. Degradation rate of immobilization grains degrading poly-containing wastewater is 83.1%.