Taking the Weifang Kite Capital Gymnasium roof for the engineering background of suspen-dome structure, and the SRB and PCDSB as the horizontal and vertical isolation equipment for the roof respectively, the S-P three-dimensional isolation layer of the roof was designed. By using subspace iteration method, the dynamic characteristics of the isolation roof were analyzed. The non-linear time history analysis procedure X based on the Newmark step-by-step integration method and the Runge-Kutta iterative method were used to solve the seismic responses of the isolated roof programmed. The validity of the procedure X was verified with ABAQUS finite element software, and the seismic responses of the non-isolation roof and the isolation roof under Ⅷ intensity and Ⅸ intensity seismic action were contrasted and analyzed with procedure X. The results have indicated that the first four vibration modes of the isolation roof are embodied for the coupling of the horizontal vibration and vertical vibration; the responses of the isolation roof are significantly less than that of the non-isolation roof under the same intensity and the same seismic action; the responses of the isolation roof of Ⅸ intensity are greater than that of Ⅷ intensity under the same seismic action; and the isolation roof designed by this method can still hold the lower seismic responses even under low frequency pulse action.