The effects of Triton X-100 on the removal of aqueous phenol catalyzed by laccase were studied. 155 μM was the optimal concentration of Triton X-100 to improve phenol removal when the concentrations of phenol and laccase were 50 mg/L and 0.05 mg/mL, respectively. Laccase activity was increased with Triton X-100 at concentrations from 31 to 930 μM and the highest increase was about 17% by 930 μM Triton X-100. The removal efficiencies of phenol with 155 μM Triton X-100 were 1.2, 1.6, 3.4, 4.5 and 5.7 times of those of the control after 6 h when the initial concentrations of phenol was 50, 100, 200, 400 and 600 mg/L, respectively. The reaction of phenol removal was also characterized by using UV spectra. The results indicated that Triton X-100 had application potential for the enhancement of phenol removal catalyzed by laccase in water treatment or remediation.