To investigate the expression pattern of human spermatogenesis associated gene 12（SPATA12）and its correlation with clinicopathological features in multiple tumors,Virtual Northern analysis based on EST in 41 human normal tissues and corresponding tumor tissues was firstly used to evaluate digital mRNA profiling of SPATA12. Then in situ hybridization was performed to detect the expression of SPATA12 in 96 cases of multiple-organ tumor tissues and 208 cases of lung tumor tissues by using tissue micro-array.Virtual Northern results show that SPATA12 is predominantly expressed in normal testis and slightly expressed in lung cancers. In situ hybridization results demonstrate that SPATA12 gene has different expression frequency in multiple tumor tissues. SPATA12 is highly expressed in the cutaneous malignant melanoma, prostatic carcinoma and chronic inflammation of prostate, gastric cancer and its cancer-adjacent tissues, prostatic carcinoma and its cancer-adjacent tissues, papillary thyroid carcinoma and pancreatic ductal adenocarcinoma. In lung tumors，the positive rates of SPATA12 expression in lung adenocarcinoma, squamous cell carcinoma, carcinoid and large cell lung cancer tissues were 16.13%,29.17%,30%,100%，respectively. There was a significant difference between SPATA12 expression and different pathological casificated lung cancers (P<0.01)，but no significant difference among age,gender and clinical stages (P>0.05). The expression pattern of SPATA12 gene is consistent with the expression feature of cancer/testis antigens family. And the expression of SPATA12 in lung tumor patients indicate that SPATA12 is closely related to pathological classification．Detection of SPATA12 might be helpful to evaluate molecular classification for lung tumors．