Microorganisms were obtained by means of separation and purification experiments from waxy oil production wells in Daqing Oilfield. The paraffin-degrading strain and the biosurfactant-producing strain were identified as Bacillus sp.. As the indicator of the paraffin degradation rate, the paraffin-degrading strain and biosurfactant-producing strain were added in different proportions. When the optimum proportion was 5∶3, the paraffin degradation rate could reach 59%, the paraffin prevention rate could reach 57.4%, the reduction rate of oil viscosity was 44.7%, the oil freezing point reduced by 3.4 ℃, and the reduction rate of culture surface tension was 46.5% after 7 days. Microbial paraffin-removal technology was used for paraffin removal in Wells 12-36, 13-39 and 14-43 in Yushulin Zone Daqing Oilfield. The results indicated that the daily crude oil increased by 41.2%, hot washing cycles prolonged from 40 d to 149d and washing numbers reduced by 4 times in Well 12-36, the daily crude oil increased by 33.3%, hot washing cycles prolonged from 45 d to 158 d and hot washing numbers reduced by 5 times in Well 13-39, the daily crude oil increased by 37.5%, hot washing cycles prolonged from 30d to 122 d and hot washing numbers reduced by 5 times in Well 14-43.