HeLa cells treated with 4.8%（V%）ethanol for 12h was used as the oxidative damage model to study the intervention of oxidative damage cells with resveratrol (RES). Varied technologies were used for analysis, such as MTT assay, flow cytometry, biochemical analysis and real time PCR et al. After the addition of RES for 24 h, the morphology of most cells was recovered, the survival rate was improved, the content of MDA was decreased from 3.56nmol/mgprot to 1.42nmol/mgprot, the T-SOD activity was increased from 61.62U/mgprot to 87.78U/mgprot and the expression of OGG1 and SIRT1 was up-regulated 2-4 times. The results indicated that RES could repair ethanol-induced oxidative damage probably by reducing MDA, improving T-SOD activity and up-regulating expression of OGG1 and SIRT1. This study can provide a theoretical basis for the application of RES.