The variations of complex shear modulus, phase angle , rutting factor and viscosity under different temperatures and different contents of bio-oil were investigated. Some problems on the ascertaining of mixing and compaction temperature on bio-binder based on the viscosity-temperature curve were discussed. This research showed that the high temperature performances of bio-binder decreased with the increase of temperature and content of bio-oil if the testing temperature was below 135 ℃. There is some aging on the bio-binder if heated too high， and the method of determining the mixing and compaction temperature on bio-binder has to be studied in the future.