Full-scale model test method was used to investigate the seismic behavior of cast in place beam-column assembly and four new precast concrete beam-to-column connections under low-cycle reversed loading. The estimation was given for the seismic performance of new precast connection on the basis of the failure mechanism on the beam-column connections, as well as hysteretic loops, ductility, energy dissipation, etc. The results indicate that, by setting additional reinforcement in the core area of the node, it can achieve the relocation of the plastic hinge at the end of beam. The hysteresis curve of the new precast connection is plumpy, and in the pre-load, the equivalent viscous damping coefficient was smaller than the cast in place connection. However, when loaded into the ultimate load, the equivalent viscous damping coefficient of precast connections was roughly equal and even exceeded cast in place connection. It has been shown that the new connection has a better energy dissipation capacity, and can meet the requirements of “strong column weak beam, stronger joint”.