Using the methods of questionnaire and field measurement, this paper analysed the characteristics of the thermal and humidity environment of every season in mid and low altitude rural tourist areas of Chongqing. By comparing the measured value with the thermal comfort limit value of the national standards, we find out that the departure degree of temperature and humidity range is small in summer and the transition season and the large in winter. Through large-scale sample questionnaire survey, we conclude that, in each season the PMVe values are +0.67 in summer, +0.32 in the transition season and -1.20 in winter. The TSV values are +0.63 in summer, -0.64 in transition season and -1.53 in winter respectively. The thermal comfort is better in summer and transition season and the worst in winter. Through further calculation of APMV and contrast with PMVe, TSV values, we find out that the most unfavorable factors to impact thermal comfort in summer, transition season and winter are indoor temperature, indoor humidity and indoor temperature respectively. Therefore, in order to improve the thermal comfort of residences in rural tourist areas, we can take the following measures: ventilation cooling in summer, adding moisture-proof material in the exterior wall in the transition season and adopting the air source heat pump with solar house or the surface water source heat pump when conditions permit.