In this research, the jute fibers were first treated with heating, alkali, silane coupling agent and isocyanate respectively, then the jute fibers fabrics were treated with hot pressing process, and finally, the jute fiber reinforced epoxy resin composites were fabricated with VARTM molding process. Scanning Electron Microscopy (SEM) analysis shows that the interface bonding of jute fibers treated with heating and alkali reinforced epoxy resin composites has not been improved, but the interface bonding of composites has been significantly improved with the jute fibers treated with silane coupling agent and isocyanate. The hot-pressing process can not only increase the jute fibers volume content in the composites but also improve the performance of composites by the jute fibers treated in advance with silane coupling agent and isocyanate. The mechanical properties of the composites show that tensile strength, modulus and flexural strength of the jute fiber treated with the silane coupling agent reinforced composite increase by 18.6%, 71.4% and 50.2%, respectively, and the tensile strength, modulus and flexural strength of the jute fiber treated with the isocyanate reinforced composite increase by 16.3%, 34.0% and 50.3%, respectively.