In order to study the mechanism of new one-way pre-stressed tendons applied to cable-pylon anchorage zone, as well as to clarify the actual stress distribution of the tendons, a full-scale model test and finite element analysis were conducted. As the construction background, Xijiang River Bridge of Guangzhou - Zhongshan - Jiangmen highway was considered. To simulate the large-tonnage inclined load of the cable, four short parallel wire cables and a reaction beam were designed and manufactured. Strains, displacements, and cracks of the structure were measured. The predictions by the finite element analysis agreed well with the test results. The linear strain distributions of the structure were measured even at 1.2 times of designed load, and cracks did not occur in the main load-resisting members. In addition, the safety coefficient 2.04 was estimated to prevent cracks, and enough prestress reserve was provided by the one-way pre-stressed tendons placed in the longitudinal direction wall.