In order to study the wind characteristics along bridge axis at multi-pylon cable-stayed bridge site with high piers in mountainous terrain, two three-dimensional anemometers and two two-dimensional anemometers were installed on the bridge deck. The time-varying mean wind speeds of the non-stationary winds were obtained by wavelet analysis method. The mean wind and turbulence characteristics measured along the bridge axis were analyzed. The results demonstrate that the mean wind speeds and wind direction changed in the same trend along the bridge axis. The turbulence characteristics measured along the bridge axis, such as turbulence intensities, gust factors, and integral scales also changed in the same trend. Meanwhile, the mean longitudinal turbulence intensities were higher than the values recommended by wind resistant design specification for highway bridges, while the values of longitudinal turbulent wind spectrum measured along the bridge axis were lower than the specification spectrum at low frequencies and close to the specification spectrum at high frequencies. The values of vertical turbulent wind spectrum measured along the bridge axis were lower than the specification spectrum at low frequencies and not close well to the specification spectrum at high frequencies.