Phenazine-1-carboxamide is a new pesticide. This paper evaluates the residue of phenazine-1-carboxamide in tomato and three kinds of soils. New methods for determining phenazine-1-carboxamide residue in these matrices were developed by high performance liquid chromatography detection. Phenazine-1-carboxamides from soil with ammonia water + ethanol + dichloromethane = 1+2+3(v/v) and from tomato with acetonitrile were exacted, followed by chromatography columns (basic aluminum) cleanup. The mean recoveries of phenazine-1-carboxamide in soil and tomato were 80.2% to 95.7% and 80.1% to 85.6% respectively, while the relative standard deviations were 2.14% to 4.17% and 4.12% to 5.17%, respectively. The minimum detectable quantity of phenazine-1-carboxamide was 0.2 ng, and the limit of detection was 0.01 mg/kg. In a whole, this method is simple and reliable, and has weak matrix effects, which satisfies the requirement of pesticide residue analysis. It is applicable to determine the phenazine-1-carboxamide residues in soil and tomato.