Modified clinoptilolite saturated with ammonia nitrogen was regenerated by microwave radiation alone and microwave-assisted regeneration solution, respectively. The study showed that the poor regeneration effect was obtained by microwave alone. The adsorption removal efficiency of ammonia onto spent zeolite after regeneration restored to 32.31% under the conditions of the power of 462 W and microwave radiation of 12 min， and the regeneration rate was only 44.88 %. The regeneration effect of the saturated zeolite was highly enhanced by adding mixed solution of NaCl and NaOH. The saturated zeolite achieved the maximum removal rate of about 71.92%, and the regeneration rate was close to 100%, when it was treated in NaCl and NaOH mixed solution concentration of 0.01 mol/L, the ratio of solid to liquid as 1∶50, the power of 700 W, and the microwave radiation of 4 min. For all the zeolites before and after modification, adsorption and regeneration were fully characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) technique. The results demonstrated that zeolites mainly exhibited cation exchange in the process of modification, adsorption and regeneration. Microwave radiation accelerated the exchange process and deepened the ion exchange equilibrium degree between NH4+ and Na+, which contributes to the NH4+ desorption from zeolite framework fast and completely.