The parameters for thermal properties of ground and grout have directly effect on the performance of ground heat exchanger (GHE). For the problem that grout thermal properties of in-situ thermal response test (TRT) need to be measured in advance and cannot be evaluated directly by calculation, this paper applied genetic algorithm to evaluate the parameters of ground and grout thermal properties simultaneously on the basis of infinite line source (ILS) model and composite media line source (CMLS) model with wall thermal resistance correction. The method was validated by in situ TRT. Compared with the measured values, the calculation relative error of borehole thermal resistance, ground thermal conductivity and diffusivity was 3.47%, 1.42% and 4.93%, respectively. The root mean square error (RMSE) of the calculated average temperature by these two models and the measured value is 0.0505 and 0.172 ℃, respectively. The results provide important reference for GHE design.