Abstract:At home and abroad,many studies of bacterial technology to remove NO and NOxhave been performed. However,the effect and mechanism of fungal were poorly studied. To improve the removal efficiency of NO and NOx,the fungi isolated and identified from the biofilm of denitrifying tower through shaking flasks experiment was studied on its nitrogen removal characteristics to nitrate (NO3 ). Expanding culture was put into the denitrify tower to study its effect on the NO and NOx removal efficiency. The fungal community structure through high throughput sequencing analysis was monitored. The results showed that the strain 3LNB through morphological,nitrogen (NO3 ) removal characteristics research,and ITS sequence analysis was identified as Fusarium solani. For up to 10.01 g/L of NO3 _N,the denitrifying rate of bacteria control group was 5.6% with nitrogen removal efficiency of 0.56 g/L,but the denitrifying rate of strain 3LNB was 15.1% with nitrogen removal efficiency of 1.51 g/L for 10 days,which proved that the strain 3LNB had strong denitrification function. The nitrogen removal rate of NO and NOxwas increased by 5.12% and 5.36% on average,respectively,after the expanding culture 400 mL (4%) of pure strain 3LNB was put into denitrifying tower,which proved that the strain 3LNB culture can improve removal rate of NO and NOxin denitrifying tower. This may be mainly related to the presence of cytochrome P450nor in fungi such as Fusarium solani.