At home and abroad,many studies of bacterial technology to remove NO and NOxhave been performed. However,the effect and mechanism of fungal were poorly studied. To improve the removal efficiency of NO and NOx,the fungi isolated and identified from the biofilm of denitrifying tower through shaking flasks experiment was studied on its nitrogen removal characteristics to nitrate (NO3 ). Expanding culture was put into the denitrify tower to study its effect on the NO and NOx removal efficiency. The fungal community structure through high throughput sequencing analysis was monitored. The results showed that the strain 3LNB through morphological,nitrogen (NO3 ) removal characteristics research,and ITS sequence analysis was identified as Fusarium solani. For up to 10.01 g/L of NO3 _N,the denitrifying rate of bacteria control group was 5.6% with nitrogen removal efficiency of 0.56 g/L,but the denitrifying rate of strain 3LNB was 15.1% with nitrogen removal efficiency of 1.51 g/L for 10 days,which proved that the strain 3LNB had strong denitrification function. The nitrogen removal rate of NO and NOxwas increased by 5.12% and 5.36% on average,respectively,after the expanding culture 400 mL (4%) of pure strain 3LNB was put into denitrifying tower,which proved that the strain 3LNB culture can improve removal rate of NO and NOxin denitrifying tower. This may be mainly related to the presence of cytochrome P450nor in fungi such as Fusarium solani.