In order to analyze the influence of construction errors on the reliability of the Reinforced Concrete frame structure，based on the on-site measurement of one real RC frame engineering，the statistical distribution of the construction error，such as the beam column section size，the thickness of protection layer，stirrup spacing and concrete compressive strength et al.，was obtained.Based on the basic theory of structural reliability and the bearing capacity limit state equation，the reliability index of the typical frame beam and frame column in the real engineering was calculated by means of the checking point method.The influence of each construction error on the component reliability index and reliability grading of reinforced concrete frame structure was also analyzed，and the parameters sensitivity of the RC frame structure reliability was discovered.The construction errors have unnegligible influence on the reliability of RC frame members.The results are conductive to the control of critical parameters and its error in the design，construction and monitoring of real engineering.