p(nBA/DEAEMA) nanoparticles of emulsion and polymeric cilia-like membranes were prepared. Two monomers of n-butyl acrylate (nBA) and 2-(diethylaminoethyl methacrylate) (DEAEMA) were polymerized by semi continuous emulsion polymerization method，and the cilia-like membranes were prepared by attaching a Velcro-like surface to partially coalesced films. Subsequently，the prepared materials were characterized by FT-IR，1HNMR，GPC，SEM，DSC combined with particle size analysis，static contact angle measurements and mechanical analysis. Finally，BECs from SD rats were used to characterize the biosecurity of the material. Correct structure of copolymer was also confirmed by FT-IR and 1HNMR. The particle size analysis of the copolymer emulsion was PDI 0.385 and Z-average was 46.48 nm，while Tg and Tf for copolymer was 33.2℃ and 47.7℃，respectively. The contact angle of polymeric films was 102.3° with water，and the surface free energy was 13.81 J·m2. Meanwhile，SEM photos showed the size of particles was 40 to 50 nm，and the height of cilia-like layer was about 5 to 10 μm. The biosecurity of this material was eventually verified by cell experiments. The copolymer cilia-like membranes can be used as a surface modification material for artificial trachea.