The removal efficiency of a Cd-resistant Aspergillus fumigatus under different concentrations of Cd in laboratory flask culture was studied,and the changes of glutathione (GSH) production in the strain were determined. The results showed that the removal rate of Cd was 55.5% when the concentration of Cd was 50 mg/L,and the concentration of GSH was 0.89 μmol/mg. The comparison of Cd removal efficiency by Aspergillus fumigatus under different conditions showed that the optimal condition was 30 ℃,pH value of 5.0,add fluid volume of 120 mL,speed of 100 r/min,and inoculation amount of 0.4 mL (1.0×107 CFU/mL). Under the optimal condition,the removal rate of Cd was 29.8% higher than that under the initial condition,and the GSH content in the culture system was increased by 0.42 μmol/mg. The changes of glutathione peroxidase (GPX) and glutathione reductase (GR) indicated that Aspergillus fumigatus could remove the heavy metal Cd in the culture system under the optimized liquid growth conditions,GSH could promote the removal of cadmium,and its detoxification mechanism could be achieved by removing the oxidative substances produced by stress by GPX and GR in vivo.