This paper selected threaded rod as the shear connectors of glued bamboo beams. Single-sided shear tests on total 6 groups of shear-slip GluBam-Concrete composite specimens were performed. The major mechanical properties such as the load-slip curves, shear bearing capacity and anti-slip stiffness were measured and then evaluated. The test results indicate that the typical failure modes of composite blocks are the splitting of GluBam due to local compression caused by the connector, and obvious plastic deformation of rod occurs in the glued-in depth. The mechanical performance of the specimens is enhanced with the increasing diameter of dowel, while the improvement efficiency descends. The diameter of rod of 18 mm is a critical value for the shear-slip behavior, and it seems to be a reasonable choice. The quality grade of threaded rod has no substantive effect on the mechanical properties and composite action. Moreover, the action of the waterproof layer at the interface is temporary, and it can be removed for improving the composite effect.