Cell penetrating peptides are small molecular peptides that can penetrate cell membranes without damaging the structure of cell membranes. These peptides are generally composed of no more than 30 amino acids. Two tumor lineage-homing cell-penetrating peptides labeled with FITC for lung cancer and liver cancer were synthesized and added to different kinds of tumor cells with different concentration gradients. The number of cells containing fluorescence was detected. The results approved that the two kinds of cell-penetrating peptides showed different cell selectivity. The fluorescence specificity of CPP33 was strong at the concentration of 10 μmol/L. The number of cells containing fluorescence was about 50% by Flow cytometer. The fluorescence specificity of CPP44 was strong at the concentration of 10 μmol/L. The number of cells containing fluorescence was about 60% by Flow cytometer. Moreover, the number of cells containing fluorescence increased with the increase of concentration, respectively. In conclusion, these tumor lineage-homing cell-penetrating peptides may provide a new method for precise delivery of anticancer molecules in vivo.