In order to investigate the influence of steel fiber content on fiber distribution and orientation in steel fiber reinforced self-compacting concrete，four kinds of concretes were prepared using four different fiber volume contents. The relationship between rheological properties of fresh concrete and fiber volume content was investigated and the fiber density in the cut planes of beam specimens was analyzed. The flow of fresh concrete with four different fiber volume contents was simulated by ANSYS CFX，where the effect of fiber volume content on the rheological properties was considered. Based on the velocity field of concrete obtained by simulation，the fiber is simplified as a number of particles that are rigidly connected and the motion of fibers is calculated by solving the rigid body dynamics equation. Through comparison between the experimental results，it is found that the proposed simulation method can determine the distribution and orientation of fiber immersed in the concrete. Furthermore，the simulation results show that the segregation degree of fiber in the vertical direction of beam decreases with the increase of fiber volume content. On the contrary，the orientation angle between fiber and beam axis increases.