In order to improve the combustion and emission performance of the ethanol-gasoline engine, the tests on an ethanol-gasoline engine were carried out with different injection strategies under typical working conditions. The effects of injection strategies and ethanol proportions on engine combustion and emissions were compared and analyzed. The results showed that the Brake Specific Fuel Consumption（BSFC） decreased first and then became stable with TCOI in advance, and decreased with the increase of the number of injections. Compared with a single injection, multiple injections reduced BSFC by up to 7%, and carbon monoxide and hydrocarbon emissions were obviously reduced, but it led to a slight increase in nitrogen oxide emissions (much less than the impact of TCOI）. After gasoline was mixed with ethanol, the combustion duration became longer, but the thermal efficiency of the engine was significantly improved due to ignition timing and early combustion center. Increasing the proportion of ethanol and TCOI can reduce the carbon monoxide concentration by up to 15%. The effect of ethanol proportion on hydrocarbon and nitrogen oxide depended on the TCOI. TCOI was a good parameter of the injection characteristics, and the impact on emissions was greater than that of ethanol proportion and number of injections. Selecting the appropriate number of injections, ethanol proportion, and TCOI can significantly improve engine thermal efficiency and reduce emissions.