As the evaluation of passengers’ comfort is not comprehensively considering the effect of the odor of Volatile Organic Compounds (VOCs) or health hazard of VOCs in a car in the present work, automobiles were taken as a kind of indoor micro-environmental system. The Weber-Fechner law was applied to establish an evaluation method of passengers’ comfort by comprehensively considering both the odor and health hazard of VOCs in a car based on the natural background concentration of typical VOCs compositions, national standards, and the olfactory threshold data. It was also the first time to quantitatively determine the influence weights of the typical VOCs compositions on passengers’ comfort and passengers’ comfort levels combined with the coefficient of variation method and triangular fuzzy theory. In addition, this paper proposed a method of quantitatively comparing passengers’ comfort in different cars based on the comment set and carried out a case study using the above proposed evaluation method. The case study indicated that，among the four types of cars，the fourth car presented the highest passengers comfort level.