The fatigue life of polypropylene fiber reinforced concrete （PFRC） under constant-amplitude fatigue uniaxial compression at multiple levels was investigated. A total of 180 specimens were made taking into consideration of two factors： tested with polypropylene fiber aspect ratios and volume fractions and aspect ratios taken into consideration， with an emphasis on the fatigue failure mode， fatigue deformation behavior， and a fatigue equation is established. The results showed that three failure modes of PFRC can be classified： splitting failure， wedge failure and shear failure. Meanwhile， the failure section of the specimen could can be divided into three zones： fatigue source zone， fatigue crack stable propagation zone and instantaneous fracture zone. In addition， the PFRC specimen with an aspect ratio of 280 and a volume fraction of 0.2% provides the highest fatigue strength among all the designed specimens， and its fatigue strength is increased by up to 33.83% when compared with plain concrete. Finally， a unified fatigue equation was developed in consideration of fiber parameter， which can concisely describe the relationship between stress level， fatigue life and fiber parameter at a certain failure probability. The research provides a reference for the future development of design specifications and analysis of PFRC structures under cycle dynamic loads.